Table 2.

Axonal pre-miRNAs.

Species	Neuron Type	Pre-miRNAs Presence	Technique	Pre-miRNAs Characterization *	Technique	Ref
Mouse	DRG	pre-miR-132	RT-qPCR	X	X	[40]
Rat	DRG	pre-miR-16, -23a, -24-1,-25, -26a, -103-1, -125b-1,-127, -134, -138-2, -185,-221, -329, -382, -433, -541	PCR	pre-miR-25, -433	ISH	[43]
Rat	SCG	pre-miR-134, -185, -204, -338	RT-qPCR	pre-miR-338	RT-qPCR from SCG axon mitochondrial fractions, imaging of exogenous labeled pre-miR-338	[44]
Xenopus laevis	RGC	pre-let-7f, -7g, pre-miR-9a-1,-9b, -9-3, -16b, -18, -19b, -26-2,-27a, -29d, -96, -100, -103-1,-124-5, -126, -129-1, -130a,-133c, -139, -140, -143, -145,-148b, -153-1, -181a-1, -181a-2,-182, -199a, -199b, -204-1, -208,-212, -214, -222, -223, -301-2,-375, -428, -455-1, -703, -1306	MiSeq sequencing, PCR, RT-qPCR	pre-miR-181a-1	Live imaging of endogenous (MB-labeled) and exogenous (cy3-labeled) pre-miR-181a-1	[45]

The subset of axonal pre-miRNAs further studied by PCR and RT-PCR are shown in bold [45]. * Axonal presence validation [43,44,45] or characterization of pre-miRNA localization [44], trafficking [45]. Abbreviations: DRG, dorsal root ganglion; SGC, superior cervical ganglia; RGC, retinal ganglion cells; ISH, in situ hybridization; MB, molecular beacon.