Figure 1.

Study layout of bleomycin-induced lung injury and longitudinal imaging. Study layout with sampling and endpoints for the imaging group (Group I) and non-imaging group (Group II). Longitudinal imaging was done by combining MRI, CT, and PET in group I, while termination of 4–5 rats occurred every week, from Group II, for tissue sample collection and analysis of collagen content by hydroxyproline assay (HXP), lysyl oxidase (LOX) activity, gene expression analyses by RT-qPCR, and histological assessment by staining of lung tissue sections.