Figure 6.

Gene expression of markers involved in lung injury and wound healing. (A) TGF-β I, (B) TGF-β II, and (C) TGF-β III were induced at later time points, similar as (D) integrin β6. The fibrosis-associated genes (E) gremlin1 and (F) α-SMA were both mainly induced at day 14. The same appeared for (G) collagen-I and (H) collagen-III, (I) LOX, (J) periostin, (K) serpine1, and (L) serpineH1. Inflammatory-associated (M) IL-1β was not expressed at late time points. (N) TIMP1 gene expression was induced early during the first days of the model, while (O) the muc1 gene was significantly upregulated with time and peaked at day 28. All data are presented as mean ± standard error of the mean (SEM). All samples from the bleomycin group are related to the mean of control and normalized to the geometric mean of two different reference genes. Each presented group/time point contains n = 4–5.