Defects in leaf development in mtpin1-1 mtpin3-1 double mutant. (A,B) the adaxial side of a fully expanded leaf in 45-day-old wild-type and mtpin1-1 mtpin3-1 at the vegetative stage. (C,D) the abaxial side of a leaf in wild-type and mtpin1-1 mtpin3-1. (E–H) scanning electron microscope (SEM) images of epidermal cells of the adaxial (E and F) and abaxial (G,H) sides of a fully expanded leaf from 45-day-old wild-type (WT) and mtpin1-1 mtpin3-1. (I,J) Phase contrast microscopy images of mesophyll cells of wild-type and mtpin1-1 mtpin3-1. (K) Measurement of epidemic cell area of the adaxial and abaxial sides of a leaf of wild-type and mtpin1-1 mtpin3-1. (L) Measurement of mesophyll cell area of wild-type and mtpin1-1 mtpin3-1. Values are the means ± SD (n = 50); * p < 0.05, *** p < 0.001. Bars = 1 cm in (A–D), 50 um in (E–H), 20 um in (I,J).