Figure 4.
Auxin accumulated in leaf and petiole in mtpin1-1 mtpin3-1. (A,B) DR5:GUS expression in adult leaf of wild-type. Close view is shown in (B). (C,D) DR5:GUS expression in adult leaf of mtpin1-1 mtpin3-1. Close view is shown in (D). (E,F) DR5:GUS expression in petiole of wild-type and mtpin1-1 mtpin3-1. (G) Measurement of free auxin content in leaf and petiole of 45-day-old wild-type (WT) and mtpin1-1 mtpin3-1. Values are the means and SD of three biological replicates. *** p < 0.001. Bars = 1 cm in (A,C), 1 mm in (B,D–F).