Table 1.
Examples of different fucoidanases and fucoidan sulfatases, including the source of catalyzed fucoidans and their mode of cleavage action.
| Enzyme Source and Accession Number in GenBank * | Enzyme Substrate (Fucoidan Source) | Mode of Cleaving Actions EC Number * | Degradation Product and Structural Features | Ref. |
|---|---|---|---|---|
| Fucoidanases | ||||
| Flavobacteriacean strain, i.e., Mariniflexilefucanivorans SW5T - FcnA: CAI47003.1 - FdlA: AAO00510.1 - FdlB: AAO00511.1 |
Pelvetia canaliculata | - endo α-(1→4) - (EC 3.2.1.212) |
→3)-α-l-Fucp, 2-OSO3−-(1→4)-α-l-Fucp-2,3-OSO3− | [82,200,209] |
|
Formosa algae (FFA1) - WP_057784217.1 |
Sargassum horneri | - endo α-(1→4) - (EC 3.2.1.212) |
→3)-α-l-Fucp, 2-OSO3−-(1→4)-α-l-Fucp-2,3-OSO3−-(1→ fragment, with insertion of →3)-α-l-Fucp, 2,4-OSO3−-(1→ | [16] |
| F. evanescens | →3)-α-l-Fucp, 2,4-OSO3−-(1→4)-α-l-Fucp-2,4-OSO3−-(1→4)-α-l-Fucp, 2-OSO3−-(1→ | [161] | ||
|
Formosa algae KMM 3553T (FFA2) - WP_057784219.1 |
F. evanescens | - endo α-(1→4) - (EC 3.2.1.212) |
→3)-α-l-Fucp, 2,4-OSO3−-(1→4)-α-l-Fucp, 2-OSO3−-(1→ and →3)-α-l-Fucp, 2-OSO3−-(1→4)-α-l-Fucp- 2-OSO3−-(1→ | [196] |
| α-l-Fucp-2-OSO3−(1→3)-α-l-Fucp-2-OSO3− and α-l-Fucp-2,3-OSO3−(1→3)-α-l-Fucp,2-OSO3− | [34] | |||
| Fucobacter marina SA-0082 | Kjellmaniella crassifolia (sulfated fucoglucuronomanna) | - endo-α-d-mannosidase - (EC 3.2.1.130) |
Trisaccharides composed of - Δ4,5GlcpUA-(1→2)-α-l-Fucp, 3-OSO3−-(1→3)-α-d-Manp, - Δ4,5GlcpUA-(1→2)-α-l-Fucp, 3- OSO3−-(1→3)-α-d-Manp, 6-OSO3−, and - Δ4,5GlcpUA-(1→2)-α-l-Fucp, 2,4-OSO3−-(1→3)-α-d-Manp, 6-OSO3− |
[210] |
| Pseudoalteromonas citrea, KMM 3296, KMM 3297, and KMM 3298 strains | L. cichorioides (20–40 kD) | - endo α-(1→3) - (EC 3.2.1.211) |
Sulfated α-l-fucooligosaccharides of 1.7–5.0 kDa and 1.3–5.0 kDa by KMM 3296 and KMM 3298 strains, respectively | [211] |
| Littorina kurila | F. distichus | - endo α-(1→3) - (EC 3.2.1.211) |
→3)-α-l-Fucp-2,4-OSO3−-(1→4)-α-l-Fucp-2-OSO3−-(1→ | [212] |
|
Wenyingzhuangia fucanilytica CZ1127T - ANW96115.1 - ANW96116.1 - ANW96098.1 - ANW96097.1 |
Isostichopus badionotus | - endo α-(1→3) - (EC 3.2.1.211) |
α-l-Fucp-(1→3)-α-l-Fucp(2,4-OSO3–)-(1→3)-α-l-Fucp(2-OSO3–)-(1→3)-α-l-Fucp(2-OSO3–) | [202] |
| Sulfatases or sulfoesterases | ||||
|
Wenyingzhuangia fucanilytica CZ1127T - SWF1: WP_068825883.1 - SWF4: WP_068828765.1 |
F. evanescens and S. horneri |
exo-2O and -3O-fucoidan sulfatase - (EC 3.1.6.B2) |
[190] | |
| Pecten maximus | A. nodosum | 2O-fucoidan sulfatase - (EC 3.1.6.B2) |
[137] | |
* whenever available.