Figure 2.

Effector/memory CD8⁺ T cell subsets in the PB of old mice. Frequency of (A) IL-7Rα⁻KLRG-1⁺ SLEC and (B) IL-7Rα⁺KLRG-1⁻ MPEC in the blood of old mice treated with PBS, NAC, Vc, or NAC+Vc. (C) Representative FACS plots of SLEC and MPEC in a control and in a Vc-treated old mouse on day 42. Frequency of (D) CD44^hiCD62L⁻ EM and (E) CD44^hiCD62L⁺ CM CD8⁺ T cells in the blood of old mice treated with PBS, NAC, Vc, or NAC+Vc. (F) Representative FACS plots of SLEC and MPEC in a control and in a Vc-treated old mouse on day 42. Blood was harvested on days 7, 14, 21, 28, 42, and 84. Data are shown as mean ±SEM. Two-way ANOVA, Tukey post-hoc test. * p < 0.05; ** p < 0.01; *** p < 0.001.