Dendritic cell subsets in the spleen. (A) Gating strategy for DC subsets. After excluding dead cells using the FVD Zombie Violet, lineage negative (lin-) CD3−NKp46−CD19− cells were considered. FACS plots showing the gating for CD11c+MHC II+ DCs, CD11c+MHC II+CD11b− classical DCs (cDC1) and CD11c+MHC II+CD11b+ DCs (cDC2) are reported. Frequency of (B) CD11c+MHC II+ DCs, (C) cDC1, (D) cDC2, and MFI of (E) CD80 in cDC1, (F) CD80 in cDC2, (G) CD40 in cDC1, and (H) CD40 in cDC2 in the spleen of young (white columns) and old (black columns) mice treated with PBS, NAC, Vc, or NAC+Vc. Data are shown as mean ± SEM. Two-way ANOVA, Tukey post-hoc test. * p < 0.05; ** p < 0.01; ***p < 0.0001.