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. 2020 Nov 20;9(11):1610. doi: 10.3390/plants9111610

Figure 2.

Figure 2

In vitro cytotoxic evaluation of T. stellata crude extract (TCE) and its metabolites. (A) The osteostromal cells were isolated from murine bone marrow and osteogenic characters were induced. The osteogenic cells were further analyzed for cytotoxic effect against T. stellata crude extract. The concentration tested from 10 μM to 500 μM. The crude extract was treated with osteogenic cells for 72 h and evaluate the cell viability using MTT reagent. (B) The major metabolites caffeic acid (CAF) was treated with osteogenic cells in concentration of 1.0 μM to 50 μM for 7 days and 14 days cultured cells. (C) Alkaline phosphatase-specific activity. (D) Total protein content. Bars represent the mean ± SD (n = 4). Statistical results are shown as * p < 0.05, Values compared between the PBS group with CAF at different concentrations.