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. 2020 Nov 27;11:6034. doi: 10.1038/s41467-020-19862-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic figure depicting the experiment where the HCRT input (from posterior “P” to anterior “A) to the dorsal raphe (DR) is disrupted. b Representative confocal image of the DR of Hcrtr1&2 double-floxed homozygous mice injected with AAV-EF1a-mCherry-IRES-Cre-WPRE and stained with mCHERRY (red) and TPH (green) antibodies. mCHERRY-positive cells (red) are highly co-localized with TPH-positive cells (green). Higher magnification (×40, right). c, d GFP is expressed after Cre recombination. TPH-positive cells (c middle red) transduced with Cre virus (d middle red) are positive for GFP (c and d, left green). Scale bars: b 100 μm, rest 20 μm.