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. 2020 Nov 27;3:718. doi: 10.1038/s42003-020-01448-5

Fig. 1. Identification and functionality of previously unannotated toxin genes within phiCD630-1.

Fig. 1

a Protein alignment of toxin CD0977.1 with the newly identified CD0904.1, CD0956.2, and CD0956.3. The hydrophobic and positively charged amino acids are indicated in red and blue, respectively. b Maps and alignment of the phiCD630-1 and phiCD630-2 genomes. The location of toxin genes in both prophages is indicated. c Growth of C. difficile 630Δerm strains harboring the pRPF185-based plasmids on BHI agar plates supplemented with Tm and with (+ATc) or without 10 ng/ml of ATc inducer (−ATc) after 24 h of incubation at 37 °C. Schematic representations of the constructs are shown.