Table 2.
Representative Support Tools for sgRNA Design.
| Tool Name | Species | Website | References |
|---|---|---|---|
| CRISPRko | Human and mouse. | https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design | [41] |
| Cas-OFFinder | Vertebrates. Insects, plants, fungi, prokaryotes, C. reinhardtii | http://www.rgenome.net/cas-offinder/ | [42] |
| CRISPOR | Vertebrates, insects, plants, fungi, prokaryotes. | http://crispor.tefor.net/ | [43] |
| E-CRISP | Vertebrates, invertebrates. Plants, fungi, protists, and C. reinhardtii. | http://www.e-crisp.org/E-CRISP/aboutpage.html | [44] |
| Off–spotter | Human, mouse, fungi | https://cm.jefferson.edu/Off-Spotter/ | [45] |
| CRISPR-GE | Vertebrates, invertebrates, plants | http://skl.scau.edu.cn/targetdesign/ | [46] |
| Benchling | Various: vertebrates. Insects, plants, fungi, prokaryotes, and C. reinhardtii | https://benchling.com/ | - |
| Deskgen | Various: vertebrates. Insects, plants, fungi, prokaryotes | https://www.deskgen.com/ | [47] |
| CHOPCHOP | Various: vertebrates. Insects, plants, fungi, prokaryotes and Chlamydomonas | https://chopchop.rc.fas.harvard.edu/ | [48] |
| CRISPR MultiTargeter | Various: vertebrates. Insects, plants, fungi, prokaryotes | http://www.multicrispr.net/ | [49] |
| sgRNA Scorer | Various: vertebrates. Insects, plants, fungi, prokaryotes. | https://crispr.med.harvard.edu/sgRNAScorerV1/ | [50] |
| CRISPRscan | Various: vertebrates. Insects, plants, fungi, prokaryotes. | http://www.crisprscan.org/ | [51] |