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. 2020 Nov 20;18(11):577. doi: 10.3390/md18110577

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Pontin knockdown reduced colony and spheroid formation abilities in T47D and BT20 cells. Pontin knockdown with siRNA attenuated colony formation by (A) T47D and (B) BT20 cells. Left panel, representative images from a portion of field; right panel, quantification of average number of migrated cells/field. Pontin knockdown with siRNA attenuated spheroid formation by (C) T47D and (D) BT20 cells. Left panel, representative images from a portion of field; right panel, quantification of average number of migrated cells/field. Data represent the mean ± SD of three independent experiments (* p < 0.05).