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. 2020 Nov 20;12(11):3457. doi: 10.3390/cancers12113457

Figure 3.

Figure 3

Osteosarcoma cell-derived extracellular-vesicles (EVs) promote accumulation of bone marrow-derived cells in the lungs. (A) Schematic illustration of the experimental setup. Mice were treated with either PBS, 143-B EVs or SAOS-2 EVs (10 µg) 2 times a week for 3 weeks after which mice were euthanized. (B) Relative mRNA expression levels of integrin alpha M gene encoding CD11b (Itgam) in bulk lung RNA in mice of indicated treatment groups. Transcript levels are normalized to Gapdh and expressed as fold change relative to control mice. n = 6, mice were pooled from two independent experiments indicated in red and black (C) Representative flow cytometric profile (left panel) and quantification (right panel) of CD11b+ cells isolated from lungs of mice from indicated treatment groups; n = 3 mice per group. (D) Representative immunofluorescence images (left panel) and quantification (right panel) of CD11b+ cells (red) and nuclei (blue) in lungs of mice from indicated treatment groups. Scale bar, 500 µm. (*, p < 0.05, **, p < 0.01, ***, p = 0.0001, ****, p < 0.0001, Bonferroni’s one-way ANOVA test).