Skip to main content
. 2020 Nov 20;10(11):186. doi: 10.3390/bios10110186

Table 3.

Current sensitive electrochemical biosensors using catalysts.

MicroRNA Catalysts/Amplification Agents Platform Substrat or Reagent Technique Linear Range LOD Real-Samples Application References
miRNA-21 ALP/DNA-linked GO–AuNPs GCE/MgO/AuNPs AAP DPV 0.1–100 fM 50 aM Spiked human serum [98]
miRNA -21 ALP/HCR AuE α-NP SWV 1 fM–100 pM 0.56 fM Spiked HEK293T cells [104]
miRNA-21 ALP/CHA-WO3-Gr GCE/WO3-Gr/AuNPs Ascorbic acid 2-phosphate DPV 0.1 fM–100 pM 0.05 fM Serum samples from breast cancer patients [111]
miRNA -21 HRP/AuNPs AuE H2O2 Amperometry 0.1 fM–100 pM 43.3 aM A549 tumor cells [113]
miRNA-155 ALP/CHA GCE/MWCNTs PtNPs Phosphate ion
Molybdophosphate anion		 DPV 10 fM–1 nM 1.64 fM Cervical cancer cells and human breast cancer cell lines [131]
miRNA-155 ALP/- SCPE/Fe3O4 AAP DPV 0.6–9 ng/mL 29 pM Spiked human serum [132]
miRNA-21 HRP/- SCPE/Fe3O4 H2O2 Amperometry 1.0–100 pM 10 aM MCF-7 cells [116]
miRNA -155 HRP/GQDs AuE TMB Amperometry 1fM–00 pM 0.14 fM Spiked human serum [118]
miRNA-21, let-7a & miRNA-31 HRP/HCR SPCE/Fe3O4 H2O2 Amperometry 1.2–100 pM 0.66 pM MCF-7 cells [133]
miRNA-21 HRP/- SPCE/Fe3O4 H2O2 Amperometry 3.0 to 100 nM 0.91 nM MCF-7 cells [134]
miRNA-21 Copper (II) complex/HCR GCME/Fe3O4 TMB DPV 100 aM–100 nM 33 aM human serum samples from breast cancer [135]
miRNA-155 Cu-MOFs/AuNPs Au-PWE Glucose DPV 1 fM–10 nM 0.35 fM Spiked human serum [120]
miRNA-21 Sn-In2O3/- AuE O2 DPV 5 pM–0.5 fM 1.92 fM A549 and HeLa cell lines [122]
miRNA-21 G-quadruplex−hemin/- AuE H2O2 DPV 0.1 fM–0.1 pM 0.04 fM Human serum samples from breast cancer [128]
miRNA-21 G-quadruplex−hemin/HCR AuE/SWCNT-ox/NDs/SWCNTs-ox/AuNPs H2O2 DPV 10 fM–1.0 nM 1.95 fM Spiked human serum [129]

Abbreviations: GCE, glassy carbone electrode; NDs, nanodiamonds; MB, magnetic beads; SWCNTs, single-walled carbon nanotubes; α-NP, α-naphtyl phosphate; AAP, ascorbic acid 2-phosphate; WO3-Gr, tungsten oxide-graphene composites; AA, ascorbic acid; ALP, alkaline phosphatase; CHA, catalytic hairpin assembly; DPV, differential pulse voltammetry; MgO, magnesium oxide; GO, graphene oxide, AuNPs, gold nanoparticles; AuE, gold electrode; HCR, hybridization chain reaction; SWV, square wave voltammetry; HEK293T cells, from human embryonic kidney 293T cells; SPCE, screen-printed carbon electrodes; H2O2, hydrogen peroxide; HRP, horseradish peroxidase; MCF-7 cells, human breast adenocarcinoma cell line; TMB, 3,3′,5,5′-tetramethylbenzidine; GQDs, graphene quantum dots; A549, human lung carcinoma cells; HeLa cells, human cervical cancer cells; Au-PWE, gold-paper working electrode; Cu-MOFs, copper-based metal–organic frameworks; G-quadruplex−hemin, Guanine-quadruplex−hemin; MDA-MB-231, human breast cancer cell lines, Sn-In2O3, tin-doped indium oxide particles. MWCNTs, multi-walled carbon nanotube, platinum nanoparticles, PtNPs; magnetic nanoparticles, Fe3O4.