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. 2020 Nov 20;12(11):3463. doi: 10.3390/cancers12113463

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of Tid1 knockdown on mtDNA copy number and mitochondrial gene expression of gastric cancer cells. (A) The effect of Tid1 knockdown on mtDNA copy number was evaluated by real-time PCR. (B–D) The effect of Tid1 knockdown on mitochondrial gene expression was evaluated by reverse transcription (RT)-real-time PCR in the (B) AGS, (C) NUGC-3, and (D) TSGH 9201 gastric cancer cells. The efficiency of Tid1 knockdown was confirmed by Western blot or RT-real-time PCR. Data are shown as the mean ± SD of at least three independent experiments. *, p < 0.05, compared with the siRNA for scramble (siScr) control group.