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. 2020 Nov 20;9(11):2510. doi: 10.3390/cells9112510

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Knockout of SIRT3 promoted Ang-II-induced recruitment and differentiation of pericytes. (A,B) Ang-II infusion significantly increased the numbers of NG2-DsRed⁺ cells, while Ang-II further increased the numbers of NG2-DsRed⁺ cells in the kidneys of SIRT3KO mouse. (n = 3/group). * p < 0.05. Data are means ± S.E.M. (C,D) Western blots showing that angiopoietins were increased in the kidneys of mice infused with Ang-II and this was further elevated by SIRT3KO (n = 3–4/group). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are means ± S.E.M. (E–G) Co-staining of NG2/TGF-β1 and DsRed/TGF-β1 showing that SIRT3KO promoted Ang-II-induced TGF-β1 production in pericytes (n = 3/group). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are means ± S.E.M. (H–J) Co-staining of NG2/FSP-1 and DsRed/FSP-1 revealing that SIRT3KO promoted Ang-II-induced pericyte differentiation into fibroblasts (n = 3/group). *** p < 0.001, **** p < 0.0001. Data are means ± S.E.M.

Knockout of SIRT3 promoted Ang-II-induced recruitment and differentiation of pericytes. (A,B) Ang-II infusion significantly increased the numbers of NG2-DsRed⁺ cells, while Ang-II further increased the numbers of NG2-DsRed⁺ cells in the kidneys of SIRT3KO mouse. (n = 3/group). * p < 0.05. Data are means ± S.E.M. (C,D) Western blots showing that angiopoietins were increased in the kidneys of mice infused with Ang-II and this was further elevated by SIRT3KO (n = 3–4/group). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are means ± S.E.M. (E–G) Co-staining of NG2/TGF-β1 and DsRed/TGF-β1 showing that SIRT3KO promoted Ang-II-induced TGF-β1 production in pericytes (n = 3/group). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are means ± S.E.M. (H–J) Co-staining of NG2/FSP-1 and DsRed/FSP-1 revealing that SIRT3KO promoted Ang-II-induced pericyte differentiation into fibroblasts (n = 3/group). *** p < 0.001, **** p < 0.0001. Data are means ± S.E.M.