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. 2020 Nov 20;9(11):2510. doi: 10.3390/cells9112510

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Knockout of SIRT3 accelerated angiotensin (Ang)-II-induced endothelial dysfunction. (A–C). Western blots showing that deficiency of SIRT3 sensitized Ang-II-induced reduction of endothelial glycolytic enzyme 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) and glucose transporter1 (GLUT1). Endothelial glycolysis was significantly reduced in the SIRT3KO mice. (n = 3–4/group) * p < 0.05, ** p < 0.01. Data are means ± S.E.M. (D,E) Western blots revealing that Ang-II infusion resulted in an increase in the expression of p47^phox and gp91^phox, while Ang-II further increased those in the kidneys of SIRT3KO (n = 3–4/group). * p < 0.05, *** p < 0.001, **** p < 0.0001. Data are means ± S.E.M. (F) Dihydroethidium staining showing that SIRT3KO enhanced Ang-II-induced reactive oxygen species (ROS) formation (n = 3/group). *** p < 0.001, **** p < 0.0001. Data are means ± S.E.M.

Knockout of SIRT3 accelerated angiotensin (Ang)-II-induced endothelial dysfunction. (A–C). Western blots showing that deficiency of SIRT3 sensitized Ang-II-induced reduction of endothelial glycolytic enzyme 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) and glucose transporter1 (GLUT1). Endothelial glycolysis was significantly reduced in the SIRT3KO mice. (n = 3–4/group) * p < 0.05, ** p < 0.01. Data are means ± S.E.M. (D,E) Western blots revealing that Ang-II infusion resulted in an increase in the expression of p47^phox and gp91^phox, while Ang-II further increased those in the kidneys of SIRT3KO (n = 3–4/group). * p < 0.05, *** p < 0.001, **** p < 0.0001. Data are means ± S.E.M. (F) Dihydroethidium staining showing that SIRT3KO enhanced Ang-II-induced reactive oxygen species (ROS) formation (n = 3/group). *** p < 0.001, **** p < 0.0001. Data are means ± S.E.M.