p-ACC/total-ACC ratio (index of ACC activity) (A), protein expression of FAS (B), and FAS activity (C) in AML12 hepatocytes incubated with 0.3 M palmitic acid (PA), with or without pterostilbene (PT) or its metabolites (PT-G and PT-S), for 18 h. The Western blot bands shown are representative of 6 samples/group. FAS protein expression was normalized by α-tubulin. Data are means ± SEM. Values not sharing a common letter (a, b and c) are significantly different (p < 0.05). In FAS protein expression: C (control group) vs. PA, p = 0.045; PA vs. PT-G, p = 0.022. In FAS activity: C vs. PA, p = 0.04; C vs. PT-G, p = 0.029; PA vs. PT; p = 0.003; PA vs. PT-G, p = 0.001; PA vs. PT-S, p = 0.039. ACC: Acetyl-CoA carboxylase; FAS; fatty acid synthase.