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. 2020 Nov 20;12(11):3465. doi: 10.3390/cancers12113465

Figure 6.

Figure 6

VRK1 promoter is hypomethylated in MYCN-amplified NB tumors. (a) Graphic representation of the VRK1 gene promoter region and colour-coded methylation levels on 16 regional CpG probes across 105 NB tumor samples. The MYCN binding site according to MYCN ChIP-seq in neuroblastoma cell lines Be(2)-C, Kelly and NGP is depicted in green with exemplary Be(2)-C MYCN ChIP-seq signal at the top. cg2668539 probe is shown in red; (b) Correlation between VRK1 expression and the methylation of the probe cg26685539 in NB tumors. Each dot represents a patient sample and is colored code according to MYCN gene expression; (c) MYCN status-dependent sensitivity (RSA) for VRK1 knockdown in 9 NB cell lines. Negative RSA values indicate viability reduction upon knockdown.