Figure 7.

Schematic assay workflow for an “active” sandwich immunoassay relying on magnetic bead labels. Step 1: electrophoretic capturing of bacterial proteotoxins on a microarray solid surface from flow-through. Step 2: active electrophoretic labeling of the captured analyte by addition of corresponding biotinylated detection antibodies. Step 3: Microscopic detection of the microarray-bound biotin labels by addition of streptavidin-coated magnetic beads in a shear-flow and magnetic field (Reprinted with permission from [149]. Copyright (2012), American Chemical Society).