Table 2.
Comparison of the analytical sensitivity of the six molecular assays.
| Strain | 3D-Based Amplification Reactions | 5′UTR Based rtRT-PCR | ||||
|---|---|---|---|---|---|---|
| RT-PCR Conventional | rtRT-PCR SYBR Green | rtRT-PCR TaqMan Probe | RT-LAMP | 2B-IR TaqMan Probe | 3-IR TaqMan Probe | |
| R1394 | 1 | 10 | 10 | 102 | 10 | 1 |
| UKG 27/72 | 1 | 10 | 10 | 102 | 10 | 1 |
| R1567 | 1 | 10 | 10 | 106.6 | 10 | Not detected |
Values indicate the detection limits expressed as TCID50, average of three replicates, observed for three reference strains: R1394 representative for the genomic sub-lineage 1, R1567 representative for the genomic sub-lineage 2, UKG 27/72 historical reference virus representative for the second antigenic group.