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. 2020 Nov 20;8(11):1832. doi: 10.3390/microorganisms8111832

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Agarose gel electrophoresis (1.2%) of PCR performed to confirm the recombination events. (a) Primer pair Int_F/Int_R. Lane 1: X. fastidiosa wild-type strain De Donno. Lane 2: pKLN59 plasmid. Lane 3: X. fastidiosa mutant strain DDgfp. (b) Primer pair Kan_F/Kan_R. Lane 1: X. fastidiosa wild-type strain De Donno. Lanes 2–4: X. fastidiosa mutant strain DDrpfF-. (c) Primer pair RpfF_F/RpfF_R. Lane 1: X. fastidiosa wild-type strain De Donno. Lanes 2–4: X. fastidiosa mutant strain DDrpfF-. (d) Primer pair RpfFMut_F/RpfFMut_R. Lane 1: X. fastidiosa wild-type strain De Donno. Lanes 2–4: X. fastidiosa mutant strain DDrpfF-. M: GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific, USA).