(A) Scheme for native ChIP-MS approach to identify proteins enriched at chromatin comprising FLAG-HA-tagged EED (FH-EED), either Y365A or WT.
(B) List of proteins enriched at the chromatin of EED cage mutant versus WT EED. Proteins highlighted in gray are core PRC2 components and previously reported PRC2 interactors.
(C and D) Heatmaps of SUZ12, 24 hr (C) and 8 days (D) after 4-OHT treatment to express WT EED, within a 20-kb window centered on the maximum value of peak signal in WT mESCs, comparing the genotypes indicated (scale: 0–5 for SUZ12).