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. Author manuscript; available in PMC: 2020 Nov 28.
Published in final edited form as: Mol Cell. 2018 Jun 21;70(6):1149–1162.e5. doi: 10.1016/j.molcel.2018.05.023

Figure 4. Deletion of MTF2 along with JARID2 Abolishes De Novo PRC2 Targeting to Chromatin.

Figure 4.

(A) Scheme for native ChIP-MS approach to identify proteins enriched at chromatin comprising FLAG-HA-tagged EED (FH-EED), either Y365A or WT.

(B) List of proteins enriched at the chromatin of EED cage mutant versus WT EED. Proteins highlighted in gray are core PRC2 components and previously reported PRC2 interactors.

(C and D) Heatmaps of SUZ12, 24 hr (C) and 8 days (D) after 4-OHT treatment to express WT EED, within a 20-kb window centered on the maximum value of peak signal in WT mESCs, comparing the genotypes indicated (scale: 0–5 for SUZ12).