| Antibodies |
| H3K27Me3 |
Cell Signaling Technology |
Cat# 9733 RRID:AB_2616029 |
| H3K27Me2 |
Cell Signaling Technology |
Cat# 9728 RRID:AB_1281338 |
| SUZ12 |
Cell Signaling Technology |
Cat# 3737 RRID:AB_2196850 |
| KDM2B |
Gift from R. Klose Lab |
N/A |
| HA-tag |
Abcam |
Cat# ab9110 RRID:AB_307019 |
| JARID2 |
In House |
N/A |
| EZH2 |
In House |
N/A |
| EZH2 |
BD |
Cat# 612666 RRID:AB_2102429 |
| EED |
In House |
N/A |
| MTF2 |
Protein Tech |
Cat# 16208-1-AP RRID:AB_2147370 |
| H2AV |
Active Motif |
Cat# 39715 RRID:AB_2616150 |
| H3K27Me2/3 |
In House |
N/A |
| Biological Samples |
| C57BL/6 (mESC with CreERT2 transgene) |
Gift from Agnel Sfeir |
N/A |
| ES-E14TG2a (E14 mESC) |
ATCC |
CRL-1821 |
| Chemicals, Peptides, and Recombinant Proteins |
| Anti-FLAG M2 affinity gel |
Sigma-Aldrich |
Cat# A2220-5ML RRID:AB_10063035 |
| Lipofectamine 2000 |
Thermo Fisher Scientific |
Cat# 11668027 |
| Deposited Data |
| Source data for gel images and graphs |
Mendeley Data |
https://data.mendeley.com/datasets/fzht5rdb4s/1 |
| ChIP-seq, RNA-seq and 4C-seq data |
This study |
GEO: GSE94431
|
| Experimental Models: Cell Lines |
| ES-E14TG2a – EED Y365A (cage-mutant) |
This study |
N/A |
| ES-E14TG2a – EED F97A (cage-mutant) |
This study |
N/A |
| ES-E14TG2a – EED Y358A (control-mutant) |
This study |
N/A |
| ES-E14TG2a – EED KO |
This study |
N/A |
| C57BL/6 – i-WT-r (inducible WT EED rescue) |
This study |
N/A |
| C57BL/6 – i-MT-r (inducible cage-mutant EED rescue) |
This study |
N/A |
| C57BL/6 – i-WT-r + 5xTetO (inserted downstream of HoxC cluster) + TetR-EZH2 (homozygous) |
This study |
N/A |
| C57BL/6 – i-WT-r + 5xTetO (inserted into a gene desert) + TetR-EZH2 (homozygous) |
This study |
N/A |
| ES-E14TG2a – i-WT-r + Evx2 deletion |
This study |
N/A |
| ES-E14TG2a – i-WT-r + promoter Evx2 deletion |
This study |
N/A |
| ES-E14TG2a – i-WT-r + control deletion |
This study |
N/A |
| C57BL/6 – i-WT-r + Evx2 deletion |
This study |
N/A |
| C57BL/6 – i-WT-r + Skor1 deletion |
This study |
N/A |
| C57BL/6 – i-WT-r + Lmx1b deletion |
This study |
N/A |
| C57BL/6 – i-WT-r + JARID2 KO |
This study |
N/A |
| C57BL/6 – i-WT-r + MTF2 KO |
This study |
N/A |
| C57BL/6 – i-WT-r + JARID2/MTF2 dKO |
This study |
N/A |
| Sf9 (Spodoptera frugiperda) Insect Cells |
Life Technologies |
Cat# 11496-015 |
| Oligonucleotides |
| CRISPR constructs, ChIP-qPCR and 4C-seq primers |
This study (Table S5) |
N/A |
| Recombinant DNA |
| pFASTBac1 |
Invitrogen |
Cat# 10-360-014 |
| pFASTBac1-6xHIS-EZH2 |
Lee et al., 2018 |
N/A |
| pFASTBac1-FLAG-EED |
Lee et al., 2018 |
N/A |
| pFASTBac1-SUZ12 |
Lee et al., 2018 |
N/A |
| pFASTBac1-RBAP48 |
Lee et al., 2018 |
N/A |
| pFASTBac1-FLAG-EED Y365A |
Lee et al., 2018 |
N/A |
| pSpCas9(BB)-2A-GFP |
Addgene |
Cat # 48138 |
| Software and Algorithms |
| ImageJ, Fiji |
NIH and Schindelin et al., 2012
|
N/A |
| Prism |
GraphPad Software |
N/A |
| Benchling |
https://benchling.com |
N/A |
| bedtools |
Quinlan and Hall, 2010 |
N/A |
| 4Cker-built |
Raviram et al., 2016 |
N/A |
| EpiProfile |
Yuan et al., 2015 |
N/A |
| Bowtie 2 |
Langmead et al., 2009 |
N/A |
| deepTools |
Ramírez et al., 2014 |
N/A |
| ‘ChIPpeakAnno’ package from Bioconductor |
Zhu et al., 2010 |
N/A |
| DEseq (R package) |
Anders and Huber, 2010 |
N/A |
