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. 2020 Nov 20;2020:6415671. doi: 10.1155/2020/6415671

Figure 3.

Figure 3

TXNIP expression was highly induced by NaBu in vitro and in vivo. (a) qPCR was performed to detect the mRNA expression of TXNIP, NLRP3, caspase 3, and GLUT1. HUVEC cells were treated with 2.5 mM NaBu for 24 hours and then used for total RNA extraction with Trizol. After the synthesis of cDNA, realtime-PCRs (qPCR) were performed with corresponding primer sets. Among those four genes, TXNIP was highly induced (~4 folds). (b) HUVEC cells were treated with designated concentration of NaBu for 48 hours and then collected for western blot analysis with anti-TXNIP antibody (∗P ≤ 0.05, ∗∗P ≤ 0.01). (c) Laser-induced choroid neovascularization stained with FITC-Isolectin IB4 was subjected to immunohistochemistry staining of TXNIP. Compared to control group, the CNV treated with NaBu has a higher TXNIP expression which costained with Isolectin IB4 but much more intense in the peripheral area of CNV region (bar: 400 μm). (d) Laser-induced CNV membrane was collected for RNA extraction and used to qPCR analysis. Laser photocoagulation highly induced the VEGFR2 expression (∗∗P ≤ 0.01).