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. 2020 Nov 24;12:12011–12020. doi: 10.2147/CMAR.S269572

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© 2020 Cai et al.

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Identification of target genes of SNHG1. (A) There were targeting binding locus between SNHG1 and miR-140-5p. (B) Relative luciferase activity - DLR assay. (C) RNA pull-down assay. (D) RNA analysis. (E) Expression of miR-140-5p in transfected KNS-81 and KALS-1 cells.

Notes: **Indicates that in terms of inter-group comparison or in comparison with the si-NC group, P<0.01; ***Indicates that in terms of inter-group comparison or in comparison with the si-NC group, P<0.001; ^#Indicates P<0.05 vs the SNHG1 group; ^##Indicates P<0.01 vs the SNHG1 group.

Abbreviations: SNHG1, small nucleolar RNA host gene 1; miR, microRNA; NC, negative control; DLR, dual-luciferase reporter; WT, wild type; Mut, mutant; IgG, immunoglobulin G; Ago2, Argonaute2.