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. 2020 Nov 24;12:12011–12020. doi: 10.2147/CMAR.S269572

Figure 6.

Figure 6

Co-transfection experiment. (A) Proliferation of KNS-81 cells transfected with SNHG1+mimics. (B) Proliferation of KALS-1 cells transfected with SNHG1+mimics. (C) Invasiveness of KNS-81 and KALS-1 cells transfected with SNHG1+mimics, and its microscopic photographs. (D) Apoptosis of KNS-81 and KALS-1 cells transfected with SNHG1+mimics and flow cytometry. (E–F) Levels of PI3K/AKT pathway-related proteins in KNS-81 and KALS-1 cells transfected with SNHG1+mimics, and their protein profiling.

Notes: *Indicates P<0.05 vs the si-NC group; **Indicates P<0.01 vs the si-NC group; ##Indicates P<0.01 vs the SNHG1 group.

Abbreviations: SNHG1, small nucleolar RNA host gene 1; miR, microRNA; NC, negative control; si, short interfering; MTT, 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide; OD, optical density; PI, propidium iodide; PI3K, phosphatidylinositol 3 kinase; AKT, protein kinase B; p-AKT, phosphorylated-protein kinase B; AMPK, AMP-activated protein kinase; p-AMPK, phosphorylated-AMP-activated protein kinase.