Figure 3.

miR-515-5p enhanced drug sensitivity in vivo. (A) Relative expression of miR-515-5p in SO-RB50 cells stably expressing miR-515-5p was detected using RT-qPCR; (B) Effect of VCR or CBP on tumor volume in nude mice; (C) A representative image of tumors in nude mice 4 weeks after injection of SO-RB50 cells stably overexpressing miR-515-5p; (D) Effect of VCR or CBP on tumor weight in nude mice; (E) Immunohistochemistry was used to detect the expression of Notch1 and Ki67 in tumor tissues of nude mice. N = 6. Data are presented as mean ± standard deviation. The t test was adopted for analysis of comparisons between two groups. One-way ANOVA was employed for the comparisons among multiple groups and Tukey’s multiple comparison test was applied for the post hoc test, **p < 0.01, ***p < 0.001.

Abbreviations: VCR, vincristine; CBP, carboplatin; RT-qPCR, reverse transcription quantitative polymerase chain reaction.