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Primer Sequence for RT-qPCR
| Gene | Sequence |
|---|---|
| miR-515-5p | F: 5ʹ-CGGGTTCTCCAAAAGAAAGCA-3’ |
| R: 5ʹ-CAGCCACAAAAGAGCACAAT-3’ | |
| U6 | F: 5ʹ-CTCGCTTCGGCAGCACA-3’ |
| R: 5ʹ-AACGCTTCACGAATTTGCGT-3’ | |
| Notch1 | F: 5ʹ-AGGCATCATGCATGTCAAAC-3’ |
| R: 5ʹ-TGTGTTGCTGGAGCATCTTC-3’ | |
| β-actin | F: 5ʹ-CTTAGTTGCGTTACACCCTTTCTTG-3’ |
| R: 5ʹ-CTGTCACCTTCACCGTTCCAGTTT-3’ |
