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. 2020 Nov 21;13(22):5277. doi: 10.3390/ma13225277

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Reactions and principles designed in the article: (1) Schiff base reaction equation of OSA and CMCS; (2) schematic diagram of prepared microfiber (CMF) preparation via electrostatic self-assembly of CM and PAA; (3) sodium alginate oxidation. (b) Identification of chemical groups: (b-1) Fehling’s solution and oxidized sodium alginate mixture; (b-2) solution of b1 was heated for 40 min, (b-3) Fourier transform infrared spectroscopy of OSA, CMCS, and OSA‑CMCS. Environmental scanning electron microscopy (ESEM) images of (c) prepared microfiber (CMF), with TEM of a single CMF (inset, upper right corner), (d) OSA‑CMCS topographic structure, (e) OSA‑CMCS‑CMF topographic structure, and (f) connective tissue under the skin of rat backs. (g) X‑ray photoelectron spectroscopy (XPS) element analysis. (h) Normal distribution of fibers in the loose connective tissue of rat backs (LCT) and of CMFs in OSA‑CMCS‑CMF.