Table 1.
Primers Name | Transcript_id | Sequence | Notes |
---|---|---|---|
IGF1 1 | XM_021091138.1 | F: GCCCAAGGCTCAGAAGGAAG | qRT-PCR |
R: GAGCAAAGGATCCTGCCAGT | |||
IGF2 2 | XM_021080621.1 | F: GTGGCATCGTGGAAGAGTGC | qRT-PCR |
R: CCAGGTGTCATAGCGGAAGAA | |||
IGF1R 3 | XM_005659775.3 | F: ACGAGTGGAGAAATCTGCGG | qRT-PCR |
R: TGAGCTTGGGAAAGCGGTAG | |||
IGF2R 4 | NM_001244473.1 | F: AGAAAGAGGTGCCGTGCTAC | qRT-PCR |
R: CCGGAGCGTGTCTATGTCTC | |||
IGFBP1 5 | NM_001195105.1 | F: ACAGCAAACAGTGCGAGACG | qRT-PCR |
R: TACTGATGGCATTTGGGGTCC | |||
IGFBP2 5 | NM_214003.1 | F: CAACCTCAAACAGTGCAAGATGT | qRT-PCR |
R: GCTGTGGTTTACTGCATCCG | |||
IGFBP3 5 | NM_001005156.1 | F: AGACGGAATACGGGCCTTGC | qRT-PCR |
R: TCTTGTCGCAGTTGGGGATG | |||
IGFBP4 5 | NM_001123129.1 | F: CAGCCCTCTGACAAGGACGA | qRT-PCR |
R: GCTCCGGTCTCGGATCTTG | |||
IGFBP5 5 | NM_214099.1 | F: CGAGCAAGCCAAGATCGAGAG | qRT-PCR |
R: AGCTTCTTTCTGCGGTCCTT | |||
IGFBP6 5 | NM_001100190.1 | F: CGCCCTCGGGGGAGAATC | qRT-PCR |
R: ATCTCAGTGTCTTGGACGCC | |||
IGFBP7 5 | XM_003129037.5 | F: TAAGCGGTGTGTGCGTGT | qRT-PCR |
R: ATGGAAGGACCTTGCTCGC | |||
ZBED6-1 6 | NM_001166552.1 | F: GCTTTGTTAGCGTCTGATGCC | qRT-PCR for ZBED6-KO 8 and ZBED6-WT 9 |
R: AGGTTACAAATTGCCCGCCA | |||
ZBED6-2 6 | NM_001166552.1 | F: GTACTAGAGCAAAGACTTCCATT | qRT-PCR for ZBED6-WT |
R:ACAAATTGCCCGCCAGGTAT | |||
ACTB 7 | XM_021086047.1 | F: GGACTTCGAGCAGGAGATGG | qRT-PCR |
R: AGGAAGGAGGGCTGGAAGAG |
1IGF1: insulin-like growth factor 1; 2IGF2: insulin-like growth factor 2; 3IGF1R: insulin-like growth factor 1 receptor; 4IGF2R: insulin-like growth factor 2 receptor; 5IGFBP1-7: insulin-like growth factor binding protein 1-7; 6ZBED6: zinc finger BED-type containing 6; 7ACTB: actin beta; 8 KO: knockout; 9 WT: wild type.