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. 2020 Nov 23;9(11):1168. doi: 10.3390/antiox9111168

Figure 5.

Figure 5

Western blot analysis of NF-KB, iNOS, and COX-2 expression in the lung tissues of male mice treated with RKs against CP. (A) The lung tissues from different experimental groups were lysed and the proteins separated by SDS–PAGE and western blotted using antisera against COX-2, iNOS, NF-KB and β-actin was used as a loading control. The bar graphs show quantitative relative levels of (B) COX-2 (C) iNOS, and, (D) NF-KB protein expression for Control, CP (150 mg/kg, i.p.), CP+RKs with different doses (25, 50, 100, and 200 mg/kg/p.o.). All values are presented as means ± SE (n = 4–6). Superscript symbols indicate a significant difference at p ≤ 0.05 using one- way ANOVA followed by the Bonferroni s test for multiple comparisons (n = 3). a Indicates significant differences from +control (Control) mice, b Indicates significant differences from CP, c Indicates significant differences from RKs (25 mg/kg/p.o.).