Figure 3.

Mononuclear cells of blood circulation, evaluated by FCM. Peripheral blood mononuclear cells (PBMC) were labelled with mAb-F, targeting the CD56, CD3, CD4, CD8, CD19, CD14, and CD16 molecules, and a viability marker. Next, all events were acquired from FACSAria II cytometer. (A) The use of a viability marker allows to limit the analysis to living cells, simply, physical parameters are obtained, such as FSC and SSC, to identify, in general, the region of lymphocytes (Lym), monocytes (Mon) and polymorphonuclear cells (PMN). (B) The use of mAb, linked to fluorochromes, makes it possible to quantify “n” fluorescences that represent the evaluation of a molecule. In our example, the expression of CD3 events distinguishes two populations, T and non-T cells. The expression of CD56 in non-T events allows us to identify Natural Killer (NK) cells, and the expression of CD19 allows us to identify B lymphocytes. To identify monocytes, the expression of CD14 is evaluated, where the analysis CD14 vs CD16 allows us to identify the three best-characterized subpopulations of monocytes: classical (c), intermediate (I) and non-classical (NC). In T events (total lymphocytes), the expression of CD4 and CD8 allows the identification of CD4 (T helper) and CD8 (cytotoxic T) lymphocytes. Finally, the expression of CD56 in CD3+ cells makes it possible to identify Natural Killer T (NKT) cells.