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Characteristics of the high-performance liquid chromatography (HPLC) system used.
| Liquid Chromatography | |
|---|---|
| Model | LC-4000 series, Jasco, Japan |
| Column | Reverse phase C 18 column (Polaris 4.6 × 250 mm) |
| Column temperature | 25 °C |
| Flow rate | 1 mL/min |
| Detection wavelength | 245 nm |
| Injection volume | 20 μL |
| Mobile phase | Methanol:acetonitrile = 75:25 (v/v) |
