Table 3.
In vitro inhibition of rainbow trout (O. mykiss) digestive proteases by increasing additions of Narbonne vetch (V. narbonensis) meals and the experimental diets per unit of activity in 30 g body weight specimen.
| Total Protease Activity Inhibition by Narbonne Vetch Meal | |||
| µg Narbonne Vetch Meal/UA * | Inhibition (%) | ||
| 50 | 26.81 ± 8.92 b | ||
| 100 | 32.17 ± 0.70 b | ||
| 200 | 42.01 ± 4.53 a | ||
| 300 | 46.35 ± 3.04 a | ||
| 400 | 48.74 ± 2.14 a | ||
| Total Protease Activity Inhibition by Experimental Feeds | |||
| µg Feed/UA | Control | A10 | A30 |
| 165 | 1.65 ± 0.66 b | 6.24 ± 3.54 b | 12.01 ± 3.32 a |
| 330 | 3.30 ± 1.31 b | 14.30 ± 4.47 a | 17.69 ± 6.69 a |
| 495 | 4.96 ± 1.98 b | 18.52 ± 4.61 a | 26.99 ± 5.51 a |
| 660 | 6.61 ± 2.46 c | 20.52 ± 1.26 b | 37.60 ± 2.26 a |
| 990 | 9.91 ± 3.97 c | 27.47 ± 1.79 b | 45.86 ± 1.29 a |
* UA = Unit of protease activity. Different superscript letters within each column (in Narbonne vetch meal data) or row (in experimental feeds data) denote significant differences among experimental groups (ANOVA, p < 0.05; n = 3).