Table 2.
Primers sequence of fluorescence quantitative gene.
| Genes Name | Primers Sequence | Recommended Melting Temperature (℃) | Product Size (bp) | |
|---|---|---|---|---|
| SGLT1 | Forward | AGAGGTCACAGTTGGAATGGC | 60 | 110 |
| Reverse | TGATTATGCTCCTGGGGTCTT | |||
| PEPT1 | Forward | GTAGACGATGGACAGCGACAC | 60 | 211 |
| Reverse | CAATGAGTTCTGCGAAAGGTT | |||
| IGF-I | Forward | CCAGCCTGCTGTTATTTCTTT | 60 | 80 |
| Reverse | CATTGCGGTTCTGTTGATAGT | |||
| BAX | Forward | CTTCCAGATGGTGAGTGAGGC | 60 | 242 |
| Reverse | GGGTTGTCGCCCTTTTCTACT | |||
| BCL-2 | Forward | ACTGCTTTCACGAACCTTTTG | 60 | 142 |
| Reverse | TTGATTTCTCCTGGCTGTCTC | |||
| SLC2A5 | Forward | ACAAGGATTCCGATGGTGATA | 60 | 113 |
| Reverse | GCAGGTCTGTCTTCCAATGTC | |||
| GLP2R | Forward | CCCCAGCACCCTGTATTCTCC | 60 | 175 |
| Reverse | ATTACTCGTGGCTGCTCGTCG | |||
| EGF | Forward | CAGACAAGTCGCCAGCAAACG | 60 | 195 |
| Reverse | GCCCTCCCACCTCCTCCAAGT | |||
| SLC2A2 | Forward | GGCACAAACAAACATTCCACT | 60 | 166 |
| Reverse | CTCAACCAGCATTTTCCAGAC | |||
| SI | Forward | TCAAACCACCGAGCATTAGGG | 60 | 125 |
| Reverse | AATGAAAAGCCAACCTGGGATA | |||
| LCT | Forward | TCTCCAGTGGCGTTGTCTTTC | 60 | 118 |
| Reverse | TGTCCTCGTCAGCCTATCAGA | |||
| MGAM | Forward | GGCCCTAAAACCACATAAAAG | 60 | 162 |
| Reverse | CTACGGTGTCCACCCCTACTA | |||
| GAPDH | Forward | GGCGTGAACCACGAGAAGTA | 60 | 141 |
| Reverse | GGCGTGGACAGTGGTCATAA |
SGLT1: sodium/glucose co-transporter-1; PEPT1: oligopeptide transporter 1; IGF-I: insulin-like growth factor I; BAX: bcl-2-associated x; BCL-2: b-cell lymphoma-2; SLC2A5: solute carrier family 2 member 5; GLP2R: glucagon-like peptide-2 receptor; EGF: epidermal growth factor; SLC2A2: solute carrier family 2 member 2; SI: sucrase-isomaltase; LCT: lactase; MGAM: maltase-glucoamylase.