Table 1.
Primary and secondary antisera used in immunohistochemistry.
| Antigen | Species | Source | Concentration |
|---|---|---|---|
| Primary Antisera | |||
| nNOS | Rabbit | Millipore (Bedford, MA, USA) |
1:2000 |
| NeuN | Mouse | Millipore | 1:200 |
| ChAT | Goat | Millipore | 1:200 |
| VIP | Mouse | Santa Cruz Biotech (Santa Cruz, CA, USA) | 1:200 |
| SOX-10 | Goat | SantaCruz | 1:500 |
| HuCD | Rabbit | SantaCruz | 1:200 |
| S100β | Rabbit | Dako (Dako, Santa Clara, CA, USA) |
Ready to use |
| GFAP | Mouse | Sigma (St Louis, MO, USA) |
1:300 |
| Secondary Antisera | |||
| Alexa Fluor 488 | Goat | Invitrogen (Carlsbad, CA, USA) |
1:333 |
| Alexa Fluor 594 | Mouse | Jackson ImmunoResearch (Ely, Cambridgeshire, UK) |
1:333 |
| Alexa Fluor 488 | Rabbit | Jackson ImmunoResearch | 1:333 |
| Anti-mouse C3 | Donkey | Jackson ImmunoResearch | 1:500 |
| Anti-rabbit-FP488 | Donkey | Interchim (Montluçon, France) |
1:200 |