Table 2.
Primer Sequences.
| Primer Name | Primer Sequence |
|---|---|
| GDNFDraIF | GTCAGATTTAAAATGGGATTCGGGCCACTTGGAG |
| GDNFSpeIR | GTCAGAACTAGTAGAGCCTCCACCTCCAGATCCTCCACCGCACTGCCACCTCCGCCGATACATCCACACCGTTTAGCGG |
| BstBIY38F | CGGGGGTTCGAAGAGTGTACCACATCCAGGCGGGCCTAC |
| BspEIY38R | GTTGTTTCCGGACGTCTTCGGAGGC |
| RED-gD-delF 1 | CCCGATCATCAGTTATCCTTAAGGTCTCTTTTGTGTGGTGCGTTCCGGTacaagtttgtacaaaaaagctgaac |
| RED-gD-delR 1 | CATCCCAACCCCGCAGACCTGACCCCCCCGCACCCATTAAGGGGGGGTATaccactttgtacaagaaagctgaac |
1 gD homology arms are specified in uppercase letters and GW cassette primer binding regions are specified in lowercase letters.