Skip to main content
. 2020 Nov 23;25(22):5476. doi: 10.3390/molecules25225476

Figure 9.

Figure 9

Effect of F-EtOAc and compound 1 on WT1 protein expression in KG-1a cells. (A) The levels of WT1 protein following treatments with F-EtOAc (4.58 μg/mL) and compound 1 (comp.1; 2.30 μg/mL) for 24, 48, and 72 h. Protein levels were evaluated using Western blotting and analysed using a scanning densitometer. The levels of WT1 were normalised using GAPDH protein levels. WT1 and GAPDH proteins following vehicle control at 24 h (No. 1), F-EtOAc at 24 h (No. 2), compound 1 at 24 h (No. 3), vehicle control at 48 h (No. 4), F-EtOAc at 48 h (No. 5), compound 1 at 48 h (No. 6), vehicle control at 72 h (No. 7), F-EtOAc at 72 h (No. 8), and compound 1 at 72 (No. 9). (B) Total cell number following treatment with F-EtOAc and compound 1 for 24, 48, and 72 h. Total cell numbers were determined using the trypan blue exclusion method. Each bar represented mean ± SD of three independent experiments performed in triplicate. Asterisk (*) denotes significant differences from the vehicle control; * p < 0.05; ** p < 0.01; *** p < 0.001.