Figure 3.

Changes in FoxO protein expression 4 days after denervation surgery. FoxO protein content in the gastrocnemius muscle at 4 days after surgery was measured by Western blotting. Percent changes in FoxO1a (a) and FoxO3a (b) protein contents were determined by comparing the DEN (denervated) and CON (control) muscles. In the WT-Tg and Hspg2^−/−-Tg mice, FoxO1a and FoxO3a protein expression increased significantly in the DEN compared to in the CON samples. The percent changes in FoxO1a and FoxO3a protein content between DEN and CON were significantly different between the WT-Tg and Hspg2^−/−-Tg mice. †, DEN significantly differed from CON within the same group (p < 0.05, n = 6). *, significant difference between the two groups (* p < 0.05, n = 6). Values are expressed as the mean ± SD. (c) The Western blotting results demonstrate the levels of FoxO1a, FoxO3a, and total protein by Ponceau S staining.