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. 2020 Nov 21;13(22):5270. doi: 10.3390/ma13225270

Figure 14.

Figure 14

Toxicity assays: (a) In vitro toxicity assays. Relative cell viability of B16, SMMC-7721, and J774A.1 cells after incubation with Mock (no treatment) and hydrogels without black phosphorus nanosheets (BPNSs) or with BPNS concentrations of 95, 190, 285, and 380 ppm for 24 h; (b,c) in vivo toxicity assays after subcutaneous injection of 100 μL of saline, hydrogel, or cellulose/BPNSs; (b) body weight was monitored on days 1, 3, 5, 7, 9, 11, 13, and 15; (c) hematoxylin and eosin (H&E) staining of the heart, liver, spleen, lung, and kidney on day 15; (d) Immunotoxicity assays in vivo for protein levels of TNFα, IL-1β, and IL-6 in the serum of C57BL/6 mice after indicated treatments [138].