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. 2020 Nov 23;9(11):2522. doi: 10.3390/cells9112522

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Results of immunodetection of β-catenin, STAT3 (Y705), STAT5α, SLUG, TWIST2, and N-cadherin by flow cytometry. Results show a significant downregulation of all analyzed proteins in both edited cells. Flow cytometry analyses show cells not incubated with primary and secondary antibody (histogram in black), cells exclusively incubated with secondary antibody (histogram in green), and cells incubated with both primary and secondary antibodies (histogram in red). There were 10,000 events analyzed in duplicate. *** p < 0.001.