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. 2020 Nov 22;21(22):8832. doi: 10.3390/ijms21228832

Figure 3.

Figure 3

Methyltransferase activity and automethylation level of Clr4 WT at different concentrations of the H3K9me0 and H3K9me1 histone peptides. Exemplary autoradiographic gel images of in vitro methyltransferase assays with Clr4 WT using 25–400 µM H3K9me0 (A) or H3K9me1 (B) as substrate. (C) Michaelis–Menten model fit of the data for the histone peptide methylation activity observed in two independent repeats of the experiments shown in panel (A,B). All values are represented relative to the enzyme activity at 400 µM H3K9me1 peptide. (D) Analysis of the inhibition of automethylation by H3K9me0 and H3K9me1 peptides detected in two independent repeats of the experiments shown in panels (A,B). Data were fitted to an inhibition model and are represented relative to Clr4 enzyme automethylation in the absence of peptide. In panels (C,D), averages are shown as squares, error bars represent the SEM and individual data points are shown as circles. The kinetic parameters and inhibition constants are listed in Table 1.