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. 2020 Nov 22;21(22):8832. doi: 10.3390/ijms21228832

Figure 4.

Figure 4

Detection of Clr4 activity on H3K9me0 and H3K9me1 histone peptides using MALDI-TOF mass spectrometry. Example of mass spectra for the different methylated histone peptides detected after 2 h or overnight (ON) methylation using Clr4 WT enzyme and 4.5 µM H3K9me0 (A) or H3K9me1 (B) in the presence of 1 mM unlabeled AdoMet. The relative fractions of histone peptides with different methylation states are indicated in the right panels. Mass spectra of the H3K9me0 and H3K9me1 histone peptides without enzyme incubation are shown in Supplementary Figure S3.