Figure 6.

AdoMet dependence of the activity of Clr4 WT and the K455R/K472R mutant. Fractions of methylated products observed with increasing AdoMet concentrations (2–50 µM) for Clr4 WT (A) or the automethylation-deficient K455R/K472R mutant (B). Data were obtained from the analysis of corresponding relative peak intensities measured by MALDI-TOF mass spectrometry in two to four independent experiments (see Supplementary Figure S5). Averages are shown as squares, error bars represent the SEM and individual data points are shown as circles. The kinetic parameters obtained in the fitting are listed in Table 2.