Table 1.
Primary antibodies used in Western blot experiments in 3xTg mice.
| Antibody | Description | Supplier (Catalogue No.) | Dilution |
|---|---|---|---|
| Egr1 | Rabbit polyclonal | Santa Cruz (sc-189) | 1:2000 |
| Egr2 | Rabbit monoclonal | Abcam (ab108399) | 1:7500 |
| PSD-95 | Rabbit monoclonal | Abcam (ab76115) | 1:2000 |
| IκBα | Rabbit monoclonal | Abcam (ab32518) | 1:1000 |
| CaMKII | Rabbit polyclonal | Santa Cruz (M-176) | 1:2000 |
| Drp1 | Mouse monoclonal | Abcam (ab156951) | 1:1000 |
| Actin | Rabbit polyclonal | Sigma (A5060) | 1:1000 |
| Total OXPHOS rodent WB antibody cocktail | Mouse monoclonal | Abcam (ab110413) | 1:1000 |
| Porin | Mouse monoclonal | Abcam (ab14734) | 1:1000 |
| pCREB (at Serine 133) | Rabbit monoclonal | Abcam (ab32096) | 1:1000 |
| CREB | Rabbit monoclonal | Abcam (ab32515) | 1:1000 |
| AT8 (ptau at Ser202 and Thr 205) | Mouse monoclonal | Thermo Fisher Scientific (MN1020) | 1:500 |
| Total tau | Rabbit polyclonal | Abcam (ab39524) | 1:1000 |
| mOC87 (amyloid fibril & APP) | Rabbit monoclonal | Abcam (ab201062) | 1:1000 |
| 4G8 (amyloid β 1–42) | Mouse monoclonal | Biolegend (800701) | 1:1000 |
| mOC64 (amyloid β 1–42) | Rabbit monoclonal | Abcam (ab201060) | 1:1000 |
| A11 (oligomeric amyloid β 1–42) | Rabbit polyclonal | Thermo Fisher Scientific (AHB0052) | 1:1000 |
Abrreviations: Egr1 = early growth response protein 1; Egr2 = early growth response protein 2; PSD-95 = postsynaptic density protein 95; IκBα = inhibitor of nuclear factor kappa b, alpha; CaMKII = calcium-calmodulin-dependent protein kinase II; Drp1 = dynamin-related protein 1; OXPHOS = oxidative phosphorylation; CREB = cAMP response element-binding protein; pCREB = phosphoCREB.