Skip to main content
. 2020 Nov 21;8(11):525. doi: 10.3390/biomedicines8110525

Figure 4.

Figure 4

12Z cells secrete increased inflammatory factors when grown as large spheroids but lose further sensitivity to TNFα. (A) Secreted inflammatory factors are increased in 12Z cells grown as large spheroids compared to monolayer. (B) Monolayer cells treated with TNFα secrete increased levels of inflammatory cytokines in a dose-response manner. (C) 12Z spheroids do not similarly respond, with only MCP1 secretion increasing after high doses of TNFα. Note the scale differs between graphs. N ≥ 3. * p < 0.05, ** p < 0.01, *** p < 0.001.