Figure 7.

Biofabrication of heterotypic spheroids. (A) Optimization of diameter for the total number of seeded cells and ratio of T-HESC to 12Z cells at 72 h post-seeding. The line indicates the tolerability range of Regenova technology. N = 3. (B) Spheroids made from 12Z and T-HESC cells self-assembled with 12Z-epithelial cells (Red) on the outside and T-HESC-stromal cells (Green) on the inside. Scale = 400 µm. 3 × 3 × 3 constructs biofabricated from heterotypic spheroids share similar self-assembly with (C) KRT-7 as an epithelial marker and (D) CD-10 as a stromal marker. Arrow = epithelial cells (12Z), arrowhead = stromal cells (T-HESC), dashed circle = presumed location of an individual spheroid. Scale = 50 µm. Inset Scale = 100 µm.